I-Feline N-terminal Pro-brain Natriuretic Peptide Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (fNT-proBNP)

[Igama Lomkhiqizo]

Igama: i-feline NT-proBNP yokuhlola isinyathelo esisodwa

[Izicaciso zokupakishwa]

Izivivinyo eziyi-10/ibhokisi

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Okujwayelekile

Ngesikhathi sokukhishwa, iphrotheni inamathele ku-BNP esebenzayo ngokomzimba (77th kuya ku-108th amino acids) kanye ne-n-terminal fragment NT-proBNP (1st kuya ku-76th amino acids). Lapho i-BNP, engama-amino acid angu-32 ubude, ikhishelwa egazini, ibophezela kuma-receptors ayo (NPRA kanye ne-NPRB) futhi ilawula umfutho wegazi ngokusebenzisa izindlela ezihlukahlukene. I-NT-proBNP, engama-amino acid angu-76 ubude, ayinawo umsebenzi webhayoloji, kodwa ngenxa yokuthi inohhafu wempilo ende kune-BNP, ifaneleka kakhulu njengenkomba yokuthola izifo ezihlukahlukene zenhliziyo. Ekuhlolweni komtholampilo kwezilwane ezifuywayo, ukugcwala kwegazi kwe-NT-proBNP ezinjeni kungaphezu kuka-900 pmol/L, futhi amakati angaphezulu kuka-270 pmol/L, futhi kunengozi enkulu yezifo ezihlobene nenhliziyo. Kodwa-ke, kufanele kuqashelwe ukuthi ngenxa yokuthi i-NT-proBNP ikhishwa yizinso, lapho isilwane sihlushwa izifo ezihlobene nezinso, ukuhlushwa kwe-NT-proBNP emzimbeni kuzophinde kwande futhi kubonakale amanga ekuhlolweni.

Isimiso Sokuthola

Lo mkhiqizo usebenzisa i-fluorescence immunochromatography ukuthola okuqukethwe kwe-fNT-proBNP ku-serum/plasma ngokobuningi. Isimiso esiyisisekelo: Kunemigqa engu-T no-C kulwelwesi lwefayibha ye-nitric acid ngokulandelana, futhi umugqa ongu-T uhlanganiswe ne-antibody a eqaphela ngokukhethekile i-fNT-proBNP. Iphedi yenhlanganisela ifuthwa ngenye i-fluorescent nanomaterial ebhalwe ukuthi i-antibody b ekwazi ukuhlonza ngokuqondile i-FDT-probNP. Kusampula, i-FDT-probNP iqala ngokuhlangana ne-nanomaterial enelebuli ye-antibody b ukuze yakhe inkimbinkimbi, bese kuba i-chromatography ephezulu, inkimbinkimbi ihlangana ne-T-line antibody A ukuze kwakheke isakhiwo sesemishi. Lapho isasasa lomsebe wokukhanya, i-nanomaterial ikhipha amasiginali we-fluorescence. Amandla esignali ahlotshaniswa kahle nokugxila kwe-fNT-proBNP kusampula.

Okwedlule: I-Canine/Feline CystatinC Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (CysC)

Olandelayo: I-Cholyglycine Quantitative Kit (i-Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (CG)

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