Ukutholwa Okuhlanganisiwe Kwezimpawu Zezempilo Ze-Canine(izinto ezi-5-6)

【Inhloso yokuhlola】
I-Canine pancreatic lipase (cPL) : I-Canine pancreatitis isifo esivuvukalayo esingena kumanyikwe.Ngokuvamile, ingahlukaniswa nge-pancreatitis eyingozi kanye ne-pancreatitis engapheli.Ukungena kwe-pancreatic neutrophil, i-pancreatic necrosis, i-peripancreatic fat necrosis, i-edema nokulimala kungabonakala ku-pancreatitis eyingozi.I-Pancreatic fibrosis kanye ne-atrophy ingabonakala ku-pancreatitis engapheli.Uma kuqhathaniswa ne-pancreatitis eyingozi, i-pancreatitis engapheli ayinabungozi kangako, kepha ivame kakhulu.Lapho izinja zihlushwa i-pancreatitis, i-pancreas yonakaliswa, futhi izinga le-pancreatic lipase egazini lenyuka kakhulu.Njengamanje, i-pancreatic lipase ingenye yezinkomba ezinhle kakhulu zokucaciswa kwe-pancreatitis ezinjeni.
I-Cholyglycine (CG) ingenye ye-conjugated cholic acid eyakhiwe yinhlanganisela ye-cholic acid ne-glycine.I-Glycocholic acid iyisakhi se-bile acid ebaluleke kakhulu ku-serum ngesikhathi sokukhulelwa sekwephuzile.Lapho amangqamuzana esibindi alimala, ukutholwa kwe-CG ngamaseli esibindi kwehla, okuholela ekwandeni kokuqukethwe kwe-CG egazini.Ku-cholestasis, ukuphuma kwe-cholic acid ngesibindi kuphazamisekile, futhi okuqukethwe kwe-CG okubuyiselwe ekujikelezeni kwegazi kuyanda, okwandisa okuqukethwe kwe-CG egazini.
I-Cystatin C ingenye yamaprotheni e-cystatin.Kuze kube manje, i-Cys C iyinto engapheli ehlangabezana ngokuyisisekelo nezidingo zomaka ophelele we-GFR.Kuyinkomba ebucayi futhi eqondile yokuhlolwa kokusebenza kwezinso ze-canine.
I-N-terminal pro-brain natriuretic peptide (Canine NT-proBNP) iyinto ekhiqizwe ama-cardiomyocyte ku-Canine ventricle futhi ingasetshenziswa njengenkomba yokuthola yokuhluleka kwenhliziyo okuhambisanayo.Ukugxila kwe-cNT-proBNP egazini kuhlobana nobukhulu besifo.Ngakho-ke, i-NT-proBNP ayikwazi nje ukuhlola ukuqina kokuhluleka kwenhliziyo okubuhlungu nokungapheli, kodwa futhi isetshenziswe njengenkomba ye-prognosis yayo.
Ingqikithi ye-Canine allergen IgE (cTIgE) :I-IgE iwuhlobo lwe-immunoglobulin (Ig) enesisindo samangqamuzana esingu-188kD nokuqukethwe okuphansi kakhulu ku-serum.Ngokuvamile isetshenziselwa ukuxilongwa kokungezwani komzimba.Ngaphezu kwalokho, kungasiza futhi ekuxilongeni izifo ze-parasitic kanye ne-myeloma eminingi.1. Ukungezwani komzimba: lapho ukungezwani komzimba kwenzeka, kuholela ekwandeni kwe-allergen ye-lgE.Uma i-allergen iphezulu i-lgE, yilapho i-aleji iyingozi kakhulu.2. Ukutheleleka kwe-parasite: ngemva kokuba isilwane singenwe ama-parasite, i-allergen ye-lgE ingase ikhule, ngokuvamile ehlobene nokungezwani okuncane okubangelwa amaprotheni e-parasite.Ngaphezu kwalokho, ukuba khona okubikiwe komdlavuza kungase kube nomthelela ekukhuphukeni kwe-IgE ephelele.

【 Umgomo wokutholwa 】
Lo mkhiqizo usebenzisa i-fluorescence immunochromatography ukuze uthole ngokomthamo okuqukethwe kwe-cPL/CG/cCysC/cNT-proBNP/cTIgE egazini le-canine.Isimiso esiyisisekelo siwukuthi ulwelwesi lwe-nitrocellulose luphawulwe ngemigqa engu-T no-C, futhi umugqa ongu-T umbozwe nge-antibody a eyiqaphela ngokuqondile i-antigen.Iphedi yokubopha ifuthwa ngenye i-fluorescent nanomaterial ebizwa ngokuthi i-antibody b ekwazi ukubona ngokuqondile i-antigen.I-antibody kusampula ibophezela ku-nanomaterial enelebuli ye-antibody b ukuze yakhe inkimbinkimbi, ebese ibophezela ku-T-line antibody A ukuze yakhe isakhiwo sesemishi.Uma ukukhanya okujabulisayo kukhanyiswa, i-nanomaterial ikhipha amasignali e-fluorescent.Ukuqina kwesiginali bekuhlotshaniswa kahle nokugxiliswa kwe-antigen kusampula.