I-Canine Progesterone Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (cProg)

[Igama Lomkhiqizo]

Igama: cProg isinyathelo esisodwa sokuhlola

[Izici Zokupakisha]

Izivivinyo eziyi-10/ibhokisi

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Inhloso Yokutholwa

Ukuhlushwa kwe-canine progesterone ku-serum kuhlobene nesiteji se-estrus yenja.Uma kuqhathaniswa ne-LH, ukugxila kwe-cProg kuhlale kukhuphuka ngesikhathi se-estrus yenja yesifazane, okulula ukuyilandelela futhi ingahlehliswa ngesikhathi sangempela Isikhathi esingcono kakhulu sokuzala siyizinsuku ezi-3-6 ngemuva kokuphakama kwe-LH, kuye ngowesifazane. isimo se-estrus yenja.Phakathi kwabesifazane abahlukene, Izinga le-progesterone elihambisana nesikhathi sokukhwelana elifanele lihluka kakhulu, ngokuvamile lisukela ku-0-50ng / ml, kodwa futhi kwakukhona okungaphezu kwalokho Kulolu hlu, ngakho-ke, izinga le-keratinization ye-epithelium yangasese yahlanganiswa ukuqapha okunamandla kwesikhathi sangempela kokugxilwa kwe-serum progesterone Indlela yokuhlola ingathuthukisa kakhulu amathuba okukhulelwa kwezinja zesifazane.

Isimiso Sokuthola

Okuqukethwe kwe-cProg ku-serum/plasma yenja kutholwe ngokomthamo nge-fluorescence immunochromatography.Isimiso esiyisisekelo: I-fiber nitrate Kukhona imigqa engu-T no-C efilimini enobukhulu ngokulandelana, futhi umugqa T umbozwe nge-cProg antigen a, ekwazi ukubona ngokuqondile i-cProg ngokufafaza kuphedi.
I-cProg kusampula yaqale yahlanganiswa ne-nanomaterial enelebuli ye-antibody b ukuze yakhe i-Complex, bese kuthi esigabeni esiphezulu, inkimbi iqhudelane ne-T-line antigen a futhi ayikwazi ukuthwebula;Kunalokho, uma lingekho isampula Ebukhoneni be-cProg, i-antibody b ibophezela ku-antigen a.Lapho i-excitation light irradiation, i-nanomaterial ikhipha isignali ye-fluorescence.Amandla esignali ahambisana ngokuphambene nokugxilisana kwe-cProg kusampula.

Umphumela Wokuhlola

Njengoba amazinga aphezulu e-progesterone ehlobene nokuzala, ubudala kanye nosayizi wenja, alikho inani eliphelele elingaguquki, izigaba ezilandelayo:
Ukuze uthole ireferensi kuphela, kunconywa ukuthi ilabhorethri ngayinye noma isibhedlela sisungule ireferensi yaso ngokuya ngomtholampilo
Akukho ekushiseni:<1ng/ml;
I-Estrus:ukuhlushwa kweprogesterone kancane kancane kuyanda, umjikelezo ngokuvamile izinsuku ezingu-7-8;Ukuze uthuthukise izinga lokuphumelela lokukhulelwa, ukuhlolwa kokuqala kwe-progesterone
Ukugxila kufanele kube phakathi kwebanga lika-10-50ng/ml, futhi kunconywa ukuzala kabili.
10-30ng/ml:ukukhwelana kokuqala kungakapheli 3h, ukukhwelana okwesibili phakathi 48h;
30-60ng/ml:ukukhwelana kokuqala phakathi kwama-2h kanye nokukhwelana kwesibili phakathi namahora angama-24;
60-80ng/ml:2h yokukhwelana.
Ibanga lokutholwa kwale kit ngu-1-80ng/ml.Uma idlula ububanzi, yazisa u-< 1ng/ml, noma > 80 ng/ml.

Okwedlule: I-Feline Total lgE Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (fTIgE)

Olandelayo: I-Canine N-terminal Pro-brain Natriuretic Peptide Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (cNT-proBNP)

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