Ukutholwa Okuhlanganisiwe Kwe-Feline Health Marker (izinto ezi-5-6)

【Inhloso yokuhlola】
I-Feline pancreatic lipase (fPL) : I-pancreas iyindlala yesibili ngobukhulu emzimbeni wesilwane (eyokuqala isibindi), etholakala esiswini esingaphambili somzimba, ihlukaniswe ngama-lobes kwesokunxele nakwesokudla. Umsebenzi wawo oyinhloko ukukhiqiza ama-enzyme adingekayo emzimbeni.I-Pancreatitis ihlukaniswe i-pancreatitis eyingozi kanye ne-pancreatitis engapheli. Umonakalo obangelwa okokuqala ungowesikhashana, kuyilapho lokhu kushiya i-fibrosis engapheli kanye ne-atrophy phakathi nokuvuvukala okungapheli okuphindaphindiwe. Phakathi kwazo, i-pancreatitis engapheli ibalelwa ku-2/3 ye-cat pancreatitis.
I-Cholyglycine (CG) ingenye ye-conjugated cholic acid eyakhiwe yinhlanganisela ye-cholic acid ne-glycine. I-Glycocholic acid iyisakhi se-bile acid ebaluleke kakhulu ku-serum ngesikhathi sokukhulelwa sekwephuzile. Lapho amangqamuzana esibindi alimala, ukutholwa kwe-CG ngamaseli esibindi kwehla, okuholela ekwandeni kokuqukethwe kwe-CG egazini. Ku-cholestasis, ukuphuma kwe-cholic acid ngesibindi kuphazamisekile, futhi okuqukethwe kwe-CG kubuyele ekujikelezeni kwegazi kuyanda, okwandisa okuqukethwe kwe-CG egazini.Ama-bile acids agcinwe ku-gallbladder, engaqedwa ngomgudu wesibindi ngemva kokudla. Ngokufanayo, izifo zesibindi nokuvinjelwa kwe-bile duct kungase kubangele inkomba engavamile.
I-Cystatin C ingenye yamaprotheni e-cystatin. Umsebenzi wokuphila obaluleke kakhulu ukulawula ukusebenza kwe-cysteine ​​​​protease, enomphumela onamandla kakhulu wokuvimbela i-cathepsin B, i-papain, i-figs protease, ne-cathepsin H futhi mina ekhishwe ngama-lysosomes. Idlala indima ebalulekile kumetabolism yama-peptide e-intracellular namaprotheni, ikakhulukazi ku-metabolism ye-collagen, engakwazi ukukhipha amanye ama-prehormones futhi awakhulule ezicubu eziqondiwe ukuze adlale indima yawo yebhayoloji. I-Hereditary cerebral hemorrhage nge-amyloidosis yisifo esihlobene ngokuqondile nokuguqulwa kofuzo lwe-cystatin C, okungabangela ukuphuka kwemithambo yobuchopho, ukopha kobuchopho neminye imiphumela emibi. Izinso ukuphela kwendawo yokususa i-cystatin C ejikelezayo, futhi ukukhiqizwa kwe-cystatin C kuhlala njalo. Izinga le-serum cystatin C lincike kakhulu ku-GFR, okuwuphawu olufanele lwe-endogenous ukukhombisa izinguquko ze-GFR. Izinguquko kokuqukethwe kolunye uketshezi lomzimba nazo zihlotshaniswa nezinhlobonhlobo zezifo.
I-NT-proBNP (i-N-terminal pro-brain natriuretic peptide), eyaziwa nangokuthi i-B-type diuretic peptide, iphrotheni ekhishwa ama-cardiomyocyte kuma-ventricles enhliziyo. Lapho umfutho wegazi we-ventricular ukhuphuka, ukuvuleka kwe-ventricular, hypertrophy ye-myocardial, noma ukucindezela ku-myocardium kuyanda, isandulela se-NT-proBNP, i-proBNP (ehlanganisa ama-amino acid angu-108), ikhishwa egazini ngama-cardiomyocyte.
Ingqikithi yokungezwani komzimba nekati IgE (fTIgE) :I-IgE iwuhlobo lwe-immunoglobulin (Ig) enesisindo samangqamuzana esingu-188kD nokuqukethwe okuphansi kakhulu ku-serum. Ngokuvamile isetshenziselwa ukuxilongwa kokungezwani komzimba. Ngaphezu kwalokho, kungasiza futhi ekuxilongeni izifo ze-parasitic kanye ne-myeloma eminingi. 1. Ukungezwani komzimba: lapho ukungezwani komzimba kwenzeka, kuholela ekwandeni kwe-allergen ye-lgE. Uma i-allergen iphezulu i-lgE, yilapho i-allergies ibucayi kakhulu. 2. Ukutheleleka kwe-parasite: ngemva kokuba isilwane singenwe ama-parasite, i-allergener LgE ingase ikhule, ngokuvamile ehlobene nokungezwani okuncane okubangelwa amaprotheni e-parasite. Ngaphezu kwalokho, ukuba khona okubikiwe komdlavuza kungase kube nomthelela ekukhuphukeni kwe-IgE ephelele.
【 Umgomo wokutholwa 】
Lo mkhiqizo usebenzisa i-fluorescence immunochromatography ukuze uthole ngokomthamo okuqukethwe kwe-fPL/CG/fCysC/fNT-proBNP/fTIgE egazini lekati. Isimiso esiyisisekelo ukuthi ulwelwesi lwe-nitrocellulose luphawulwe ngemigqa engu-T no-C, futhi umugqa ongu-T umbozwe nge-antibody a eqaphela ngokukhethekile i-antigen. Iphedi yokubopha ifuthwa ngenye i-fluorescent nanomaterial ebizwa ngokuthi i-antibody b ekwazi ukubona ngokuqondile i-antigen. I-antibody kusampula ibophezela ku-nanomaterial enelebuli ye-antibody b ukuze yakhe inkimbinkimbi, ebese ibophezela ku-T-line antibody A ukuze yakhe isakhiwo sesemishi. Uma ukukhanya okujabulisayo kubayisiwe, i-nanomaterial ikhipha amasignali e-fluorescent. Ukuqina kwesiginali bekuhlotshaniswa kahle nokugxiliswa kwe-antigen kusampula.