Ipheshana lokuphefumula le-Canine Ukutholwa Okuhlanganisiwe (izinto ezi-4)


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

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Imininingwane yokupakisha

I-Canine Distemper Virus (CDV) ingeyohlobo lwe-Measles virus yomndeni we-Paramucosal virus, engabangela ukusabalala kwezifo ezithathelwanayo ze-canine (i-canine distemper) futhi iholele ezimweni zomtholampilo ezifana ne-conjunctivitis, inyumoniya kanye ne-gastroenteritis ezinjeni, njll. I-distemper virus ibonakala ngokufa okuphezulu, ukusuleleka okunamandla kanye nezifo ezifushane.Ikakhulukazi phakathi kwemidlwane, kunezinga eliphezulu lokutheleleka nokufa.
I-Canine adenovirus type II ingabangela i-laryngotracheitis ethathelwanayo kanye nezimpawu ze-pneumonia ezinjeni.Izici zomtholampilo zihlanganisa umkhuhlane ophezulu ongapheli, ukukhwehlela, i-serous to mucinous rhinorrhea, i-tonsillitis, i-laryngotracheitis, ne-pneumonia.Ngokwezibalo zezigameko zomtholampilo, lesi sifo sivame kakhulu emidlwaneni engaphansi kwezinyanga ezi-4.Udoti - noma ukukhwehlela kweqembu lonke kungabangelwa emidlwaneni, ngakho-ke lesi sifo sivame ukubizwa ngokuthi "ukukhwehlela kwe-kennel" ngokwezimpawu zomtholampilo.
I-Canine influenza ibangelwa kakhulu izinhlobo zegciwane lomkhuhlane A ikakhulukazi i-H3N8 ne-H3N2.Izimpawu zokuqala zifana kakhulu ne-bronchitis ye-kennel.Kuqala ngokukhwehlela okungapheli okungathatha amasonto amathathu futhi kuhambisane nokuphuma okuphuzi emakhaleni.
Ukutholwa okunokwethenjelwa nokuphumelelayo kunendima eyisiqondiso esihle ekuvimbeleni nasekuxilongweni nasekwelashweni.

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Isimiso Sokuthola

Umkhiqizo wasetshenziselwa ukutholwa komthamo we-CDV/CAV-2/FluA Ag esweni le-canine, ekhaleni nasemlonyeni nge-fluorescence immunochromatography.Isimiso esiyisisekelo: I-nitro fiber membrane imakwe ngemigqa engu-T no-C ngokulandelana, futhi imigqa engu-T ihlanganiswe namasosha omzimba a1, a2 kanye ne-a3 aqaphela ngokukhethekile ama-antigen e-CDV/CAV-2/FluA.Ama-antibody b1, b2 kanye ne-b3 alebulwe ngenye i-nanomaterial ye-fluorescent ekwazi ukubona ngokuqondile i-CDV/CAV-2/FluA afafazwe kuphedi yokubopha.I-CDV/CAV-2/FluA kusampula iqale yahlanganiswa nama-nanomaterial anelebula amasosha omzimba okuthi b1, b2 kanye ne-b3 ukuze kwakheke inkimbinkimbi, bese iya kungqimba olungaphezulu.I-complex ihlanganiswe namasosha omzimba we-T-line a1, a2 kanye ne-a3 ukuze kwakheke isakhiwo sesemishi.Uma ukukhanya okujabulisayo kufakwe imisebe, i-nanomaterial ikhipha isignali ye-fluorescence, futhi amandla esignali ahlotshaniswa kahle nokugxiliswa kwegciwane elincike kusampula.


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