I-Feline Total lgE Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (fTIgE)

[Igama Lomkhiqizo]

cTIgE isinyathelo esisodwa sokuhlola

 

[Izici Zokupakisha]

Izivivinyo eziyi-10/ibhokisi


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

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Inhloso Yokutholwa

I-IgE iyikilasi le-immunoglobulin (Ig) enesisindo samangqamuzana esingu-188kD nokuqukethwe okuphansi kakhulu ku-serum.Kuvame ukusetshenziswa Ekuxilongeni ukusabela okweqile, ngaphezu kwalokho, kungasiza futhi ekuxilongeni ukutheleleka kwe-parasitic, i-myeloma eminingi.1. Ukuzwela Okudlulayo: Uma kukhona ukungezwani komzimba, okuholela ekwandeni kwe-allergen ye-lgE, ukuphakama kwe-lgE ye-allergen, okubonisa ukungezwani komzimba Uma kufanele kube kubi kakhulu.2. Ukutheleleka kwe-parasite: Ngemva kokuba isilwane singenwe yi-parasite, i-allergen ye-lgE ingase ikhule.Ngokuvamile kuhlotshaniswa nokungezwani komzimba okuncane okubangelwa amaprotheni ezinambuzane.Ngaphezu kwalokho, ukwenzeka okubikiwe kwamathumba kungase kuholele ekutheni i-IgE iphelele.

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Isimiso Sokuthola

Okuqukethwe kwe-cTIgE ku-serum/plasma kutholwe ngokomthamo nge-fluorescence immunochromatography.Izimiso eziyisisekelo:
Imigqa engu-T kanye no-C yadwetshwa kulwelwesi lwefayibha ye-nitrate ngokulandelana, futhi imigqa engu-T yahlanganiswa ne-antibody a eqaphela ngokuqondile i-cTIgE antigen.Iphedi yafafazwa ngenye i-fluorescent nanomaterial ebhalwe ukuthi i-antibody b, ekwazi ukubona ngokuqondile i-cTIgE.I-cTIgE iqale iboshelwe ku-nanomaterial ebhalwe ukuthi i-antibody b ukuze yakhe inkimbinkimbi, bese kuba ungqimba olungaphezulu, i-antibody eyinkimbinkimbi kanye ne-T-line ibopha ukuze yakhe isakhiwo sesemishi.Lapho ukukhanya okujabulile kucwebezela, i-nanomaterial ikhipha isignali ye-fluorescence.
Amandla esignali ahlotshaniswa kahle nokugxila kwe-cTIgE kusampula.


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