I-Canine Cortisol (cCortisol) iyi-hormone ye-steroid ekhiqizwa i-canine adrenal cortex.I-Cortisol isiza ukugcina izinga likashukela egazini njalo, yehlisa ukuvuvukala futhi isiza umzimba ukuthi ulawule ukucindezeleka.Nakuba izimo ezihlukahlukene ezibangelwa amazinga aphezulu ngokungavamile e-hormone yotshwala zibizwa ngokuthi i-Cushing syndrome (CS), zombili izinja namakati zingaphathwa yi-CS, evame kakhulu ezinjeni kunasemakati.Izinja ezineminyaka ephakathi nendawo ubudala (cishe iminyaka engu-7 kuya kwengu-12 ubudala)
maningi amathuba okuthi ahlakulele lesi sifo.Lesi sifo sikhula kancane futhi izimpawu zokuqala akulula ukuzibona.I-CS ingatholwa ngokomtholampilo ngokuhlolwa kokuvuselela ihomoni ye-adrenocorticotropic (ACTH) kanye nokuhlolwa kokucindezelwa kwe-dexamethasone, nezinhlobo zayo ezihlukene: i-adrenal-dependent (ATH) ne-pituitary-dependent (PDH).
Lo mkhiqizo usebenzisa i-fluorescence immunochromatography ukuthola ngokomthamo okuqukethwe kwe-cCortisol ku-serum/plasma yezinja.Isimiso esiyisisekelo: Imigqa engu-T no-C imakwe kulwelwesi lwe-nitrocellulose, umugqa ongu-T umbozwe nge-cCortisol antigen a, futhi iphedi yokubopha ifuthwa nge-fluorescent nanomaterial ebhalwe ukuthi i-antibody b ekwazi ukubona ngokuqondile i-cCortisol.
I-cCortisol kusampula ibhalwe okokuqala nge-nanomaterial.I-antibody b ibopha ukuze yakhe inkimbinkimbi, bese kuba ama-chromatographs ukuya phezulu.I-complex iqhudelana ne-T-line antigen a futhi ayikwazi ukuthunjwa;Ngokuphambene, uma ingekho i-cCortisol kusampula, i-antibody b ibophezela ku-antigen a.Uma ukukhanya kwenjabulo kufakwe imisebe, okubalulekile kwe-nano kukhipha isignali ye-fluorescent, futhi amandla esignali ahambisana ngokuphambene nokugxila kwe-cCortisol kusampula.
Selokhu yasungulwa, imboni yethu ibilokhu ithuthukisa imikhiqizo esezingeni lomhlaba ngokulandela isimiso
yekhwalithi kuqala.Imikhiqizo yethu izuze idumela elihle kakhulu embonini kanye nokwethenjwa okubalulekile phakathi kwamakhasimende amasha namadala..