Ukuthola Uhudo Oluhlanganisiwe Lwe-Canine (izinto ezingu-7-10)

【Inhloso yokuhlola】
I-Canine Parvovirus (CPV) ingeyohlobo lwe-parvovirus yomndeni we-parvoviridae futhi ibangela izifo ezithathelwanayo ezinzima ezinjeni.Ngokuvamile kunezibonakaliso ezimbili zomtholampilo: uhlobo lwe-hemorrhagic enteritis kanye nohlobo lwe-myocarditis, kokubili okunezici zokufa okuphezulu, ukusuleleka okunamandla kanye nesifo esifushane, ikakhulukazi ezinjeni ezincane, ezinezinga eliphezulu lokutheleleka kanye nokufa.
ICanine Coronavirus (CCV) ingeyohlobo lwe-coronavirus emndenini iCoronaviridae futhi iyisifo esithathelwanayo esiyingozi kakhulu ezinjeni.Izimpawu ezijwayelekile zomtholampilo kwakuyizimpawu ze-gastroenteritis, ikakhulukazi ukuhlanza, isifo sohudo kanye ne-anorexia.
ICanine rotavirus (CRV) ingeyohlobo lweRotavirus yomndeni wakwaReoviridae.Ilimaza kakhulu izinja ezisanda kuzalwa futhi ibangele izifo ezithathelwanayo ezibonakala ngohudo.
I-Giardia (GIA) ingabangela isifo sohudo ezinjeni, ikakhulukazi izinja ezincane.Ngokukhula kweminyaka kanye nokwanda kokuzivikela komzimba, nakuba izinja zithwala igciwane, zizobonakala zingabonakali.Kodwa-ke, lapho inani le-GIA lifinyelela inombolo ethile, isifo sohudo sisazokwenzeka.
I-Helicobacterpylori (HP) igciwane le-gram-negative elinamandla okusinda futhi lingaphila endaweni ene-asidi eqinile yesisu.Ukuba khona kwe-HP kungase kubeke izinja engozini yohudo.
Ngakho-ke, ukutholakala okuthembekile nokusebenzayo kunendima enhle yokuqondisa ekuvimbeleni, ekuxilongweni nasekwelashweni.

【 Umgomo wokutholwa 】
Lo mkhiqizo usetshenziselwa ukuthola ngokomthamo okuqukethwe kwe-CPV/CCV/CRV/GIA/HP endle yenja nge-fluorescence immunochromatography.Isimiso esiyisisekelo siwukuthi ulwelwesi lwe-nitrocellulose luphawulwe ngemigqa engu-T no-C, futhi umugqa ongu-T umbozwe nge-antibody a eyiqaphela ngokuqondile i-antigen.Iphedi yokubopha ifuthwa ngenye i-fluorescent nanomaterial ebizwa ngokuthi i-antibody b ekwazi ukubona ngokuqondile i-antigen.I-antibody kusampula ibophezela ku-nanomaterial enelebuli ye-antibody b ukuze yakhe inkimbinkimbi, ebese ibophezela ku-T-line antibody A ukuze yakhe isakhiwo sesemishi.Uma ukukhanya okujabulisayo kukhanyiswa, i-nanomaterial ikhipha amasignali e-fluorescent.Ukuqina kwesiginali bekuhlotshaniswa kahle nokugxiliswa kwe-antigen kusampula.