Ukutholwa Okuhlanganisiwe Kwesifo Sohudo (izinto ezingu-7-10)

【Inhloso yokuhlola】
I-feline panleukopenia , eyaziwa nangokuthi i-feline distemper noma i-feline infectious enteritis, isifo esithathelwana kakhulu ngegciwane. I-pathogenic Feline parvovirus (FPV) ingeyomndeni wakwa-Parvoviridae futhi ihlasela ikakhulukazi izinsikazi. Igciwane lesifo sekati lizokwanda lapho iseli lihlanganisa i-DNA, ngakho igciwane lihlasela amaseli noma izicubu ezinekhono eliqinile lokuhlukanisa. I-FPV isakazwa kakhulu ngokumunca noma ngokuhogela izinhlayiya zegciwane ngokuthintana, kodwa futhi ingasakazwa yizinambuzane ezimunca igazi noma amazeze, noma isakazwe ngokuqondile isuka egazini noma i-placenta yekati lesifazane ekhulelwe iye embungwini.
I-Feline Coronavirus (FCoV) iyingxenye ye-coronavirus yomndeni i-Coronaviridae futhi iyisifo esithathelwanayo esiyingozi emakatini. Ama-cat coronaviruses avame ukuhlukaniswa abe izinhlobo ezimbili. Enye i-enteric coronaviruses, ebangela isifo sohudo kanye nendle ethambile. Enye i-coronavirus ekwazi ukubangela i-peritonitis ethathelwanayo emakati.
I-Feline rotavirus (FRV) ingeyomndeni we-Reoviridae kanye nohlobo lwe-Rotavirus, ebangela ikakhulukazi izifo ezithathelwanayo ezibonakala nesifo sohudo. Ukutheleleka nge-Rotavirus emakati kuvamile, futhi amagciwane angahlukaniswa endle kokubili amakati anempilo kanye nesifo sohudo.
I-Giardia (GIA) :I-Giardia isakazwa kakhulu ngomzila wendle nowomlomo. Lokhu okubizwa ngokuthi "indle-ngomlomo" akusho ukuthi amakati atheleleka ngokudla indle yamakati anegciwane. Kusho ukuthi uma ikati lizikhulula, kungase kube nama-cysts athathelwanayo esitulweni. Lama-cysts akhishwe angaphila izinyanga endaweni futhi atheleleka kakhulu, nama-cyst ambalwa kuphela adingekayo ukuze abangele ukutheleleka emakatini. Kukhona ingozi yokutheleleka lapho indle equkethe i-cyst ithintwa elinye ikati.
I-Helicobacterpylori (HP) igciwane le-gram-negative elinamandla okusinda futhi lingaphila endaweni ene-asidi enamandla yesisu. Ukuba khona kwe-HP kungase kubeke amakati engcupheni yohudo.
Ngakho-ke, ukutholakala okuthembekile nokusebenzayo kunendima enhle yokuqondisa ekuvimbeleni, ekuxilongweni nasekwelashweni.

【 Umgomo wokutholwa 】
Lo mkhiqizo usebenzisa i-fluorescence immunochromatography ukuze uthole ngokomthamo okuqukethwe kwe-FPV/FCoV/FRV/GIA/HP endle yekati. Isimiso esiyisisekelo siwukuthi ulwelwesi lwe-nitrocellulose luphawulwe ngemigqa engu-T no-C, futhi umugqa ongu-T umbozwe nge-antibody a eyiqaphela ngokuqondile i-antigen. Iphedi yokubopha ifuthwa ngenye i-fluorescent nanomaterial ebizwa ngokuthi i-antibody b ekwazi ukubona ngokuqondile i-antigen. I-antibody kusampula ibophezela ku-nanomaterial enelebuli ye-antibody b ukuze yakhe inkimbinkimbi, ebese ibophezela ku-T-line antibody A ukuze yakhe isakhiwo sesemishi. Uma ukukhanya okujabulisayo kubayisiwe, i-nanomaterial ikhipha amasignali e-fluorescent. Ukuqina kwesiginali bekuhlotshaniswa kahle nokugxiliswa kwe-antigen kusampula.