Amandla amahlanu:
● Insimbi ecushwe nge-nucleic acid kanye nesinyathelo esihlanziwe
● Insimbi ecushwe nge-ultrasonic isizinda module
● Insimbi ecushwe ngokuzenzakalelayo ngokugcwele
● Insimbi ecushwe ngezinga lokushisa eliguquguqukayo
● Insimbi ecushwe ngekhithi ye-reagent evalelwe ngokugcwele
1.Ingabe izisetshenziswa zokuthola i-nucleic acid zidinga ukukhishwa futhi zihlanzwe?
Umgomo wokutholwa kwe-nucleic acid umi kanje: ngaphansi kwesenzo se-primer, i-DNA polymerase isetshenziselwa ukwenza i-chain reaction amplification kusifanekiso se-DNA/RNA (edinga ukubhalwa okuhlanekezelwe kwe-NA), bese kutholwa inani lesiginali ye-fluorescent ekhishiwe ukuze kunqunywe. ukuthi isampula iqukethe i-nucleic acid (DNA/RNA) ye-pathogen ezotholwa.
1)Amasampula angazange akhishwe noma acwengiwe angase aqukathe izingxenye eziningi ezithinta umphumela wokugcina: i-nuclease (engancibilikisa i-nucleic acid ehlosiwe futhi ibangele okungalungile okungamanga), i-protease (enganciphisa i-DNA polymerase futhi ibangele i-negative yamanga), i-heavy metal. usawoti (okuholela ekungasebenzini kwe-synthase futhi ubangele okungelona iqiniso), i-acidic kakhulu noma ene-alkaline kakhulu PH (okungase kubangele ukusabela kuhluleke), i-RNA engaphelele (okuholela ekuhlulekeni kokuloba okunegethivu okungamanga).
2)Amanye amasampula kunzima ukukhulisa ngqo: I-Gram-positive kanye nezinye izimuncagazi, ngenxa yezindonga zawo eziwugqinsi zamangqamuzana nezinye izakhiwo, uma zingadluli kwinqubo yokukhipha i-nucleic acid kanye nenqubo yokuhlanzwa, ikhithi engena-extraction ingase yehluleke kulokho. amasampula.
Ngakho-ke, kunconywa ukuthi ukhethe ikhithi yokuhlola noma insimbi elungiselelwe ngesinyathelo sokukhipha i-nucleic acid.
2. Ukukhishwa kwamakhemikhali noma ukukhishwa kwe-ultrasonic fragmentation?
Ngokuvamile, ukukhishwa kwamakhemikhali kungasetshenziswa ezinhlobonhlobo eziningi zokwelashwa kwangaphambili kanye nokuhlanzwa.Kodwa-ke, kumabhaktheriya ane-Gram-positive anezindonga eziwugqinsi kanye nezinye izimuncagazi, kuphinde kube yisimo lapho ukukhipha kwamakhemikhali akukwazi ukuthola izifanekiso ze-nucleic acid ezisebenzayo, okuholela ekutholeni okungelona iqiniso.Ngaphezu kwalokho, isizinda samakhemikhali sivame ukusebenzisa ama-agent aqinile, uma i-lution ingaphelele, kulula ukwethula i-alkali eqinile ohlelweni lokusabela, okuholela emiphumeleni engalungile.
Ukuqhekeka kwe-ultrasonic kusebenzisa ukuchotshozwa ngokomzimba, okusetshenziswe ngempumelelo yi-GeneXpert, ibhizinisi elihamba phambili emkhakheni we-POCT ukuze lisetshenziswe abantu, futhi kunenzuzo enkulu ekukhishweni kwe-nucleic acid kwamanye amasampuli ayinkimbinkimbi (njenge-Mycobacterium tuberculosis).
Ngakho-ke, kunconywa ukuthi ukhethe ikhithi yokuhlola noma insimbi elungiswe ngesinyathelo sokukhipha i-nucleic acid.futhi ilungile uma kunemojula yokukhipha i-ultrasonic.
3. Imanuwali, i-Semi-automatic futhi i-othomathikhi ngokugcwele?
Lokhu kuyinkinga yezindleko zabasebenzi kanye nokusebenza kahle.Njengamanje, izibhedlela ezifuywayo ezingenabasebenzi abenele, kanye nokukhipha nokutholwa kwe-nucleic acid kuwumsebenzi odinga amakhono athile nolwazi.Akungabazeki ukuthi umshini wokukhipha nokuhlonza i-nucleic acid othomathikhi ngokuphelele uyisinqumo esihle.
4. Ukukhulisa izinga lokushisa okungaguquki noma ukukhuliswa kwezinga lokushisa okuguquguqukayo?
Ukusabela kwe-amplification isixhumanisi sokutholwa kwe-nucleic acid, futhi ubuchwepheshe obuchwepheshile obuhilelekile kulesi sixhumanisi bunzima.Ngokuvamile, ama-enzyme asetshenziselwa ukukhulisa i-nucleic acid.Enqubweni yokukhulisa, kutholwa isignali ye-fluorescence ekhulisiwe noma isignali eshumekiwe ye-fluorescence.Ngokuvamile, uma kuqala isignali ye-fluorescence ibonakala, kukhulu okuqukethwe kofuzo okuhlosiwe kwesampula.
Ukukhulisa izinga lokushisa okungaguquki ukukhuliswa kwe-nucleic acid ezingeni lokushisa elingaguquki, kuyilapho ukukhulisa izinga lokushisa okuguquguqukayo kuwukukhuliswa kwe-cyclic ngokuqinile ngokuya nge-denaturation-annealing-extension.Isikhathi sokukhulisa izinga lokushisa esiqhubekayo senziwe, kuyilapho isikhathi sokukhulisa izinga lokushisa esiguquguqukayo sithinteka kakhulu izinga lokushisa lokukhuphuka nokuwa kwensimbi (okwamanje, abakhiqizi abaningi bakwazi ukwenza imijikelezo ye-40 yokukhulisa cishe imizuzu engama-30).
Uma izimo zaselabhorethri zizinhle futhi ukuklanywa kwezindawo kuqinile, kunengqondo ukusho ukuthi umehluko wokunemba phakathi kwalokhu kokubili ngeke ube mkhulu.Kodwa-ke, ukukhuliswa kwezinga lokushisa okuguquguqukayo kuzohlanganisa imikhiqizo eminingi ye-nucleic acid ngesikhathi esifushane.Ezilabhorethri ezingenazo izindawo eziqinile kanye nabasebenzi abaqeqeshelwa umsebenzi, ingozi yokuvuza kwe-nucleic acid aerosol izoba nkulu, okungelona iqiniso kwenzeka uma ukuvuza sekuphelile, futhi okunzima kakhulu ukukuqeda.
Ukwengeza, ukukhuliswa kwezinga lokushisa okungaguquki nakho kuthambekele kakhudlwana ekwandiseni okungaqondile lapho isampula liyinkimbinkimbi (izinga lokushisa elihlobene lokusabela liphansi, futhi ukuphakama kwezinga lokushisa lesandiso, kuba ngcono ukucaciswa kokubopha kokuqala).
Ngokuphathelene nobuchwepheshe bamanje, ukukhuliswa kwezinga lokushisa okuguquguqukayo kunokwethenjelwa kakhulu.
5. Ungayigwema kanjani ingozi yokuvuza kwemikhiqizo yokukhulisa i-nucleic acid?
Njengamanje, abakhiqizi abaningi bakhetha i-gland yohlobo lwe-PCR tube njenge-nucleic acid reaction tube, evalwe ukungqubuzana, futhi ukushintshwa kwezinga lokushisa ekushintsheni kwezinga lokushisa eliguquguqukayo ekukhuliseni okuguquguqukayo kwe-PCR kufinyelela ku-90 degree.
I-Centigrade .Inqubo ephindaphindwayo yokwandisa ngokushisa kanye nokufinyela kubanda kuyinselele enkulu ekubekweni uphawu kweshubhu le-PCR, futhi ishubhu ye-PCR yohlobo lwegland kulula ukukubangela ukuvuza.
Kungcono ukusebenzisa ukusabela ngekhithi/ishubhu evalwe ngokuphelele ukuze ugweme ukuvuza komkhiqizo wokusabela .Kungaba kuhle uma kungaba khona ikhithi evalwe ngokugcwele yokukhipha nokutholwa kwe-nucleic acid.
Ngakho-ke umshini omusha we-nucleic acid wokutholwa ozenzakalelayo omusha we-New Tech unezinketho ezingenhla ezinhlanu ezifanele.
Isikhathi sokuthumela: Aug-09-2023
